| 质粒名称: | pEGFP-NMHC II-C |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 10843 |
| 存储实验室: | Robert Adelstein |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-N3 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4700 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | NMHC II-C,nonmuscle myosin II-C heavy chain |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 6400 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | EGFP（主干上的C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SalI(未破坏) |
| 3' 克隆位点: | BamHI(未破坏) |
| 5' 测序引物 | EGFP-N |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pEGFP-NMHC II-C was a gift from Robert Adelstein (Addgene plasmid # 10843 ; http://n2t.net/addgene:10843 ; RRID:Addgene_10843) |
| 参考文献部分: | Identification and characterization of nonmuscle myosin II-C, a new member of the myosin II family. Golomb E, Ma X, Jana SS, Preston YA, Kawamoto S, Shoham NG, Goldin E, Conti MA, Sellers JR, Adelstein RS. J Biol Chem. 2004 Jan 23. 279(4):2800-8. 10.1074/jbc.M309981200 PubMed 14594953 |
买家导航
pEGFP-NMHC II-C载体质粒基本信息
pEGFP-NMHC II-C质粒图谱载体图谱和pEGFP-NMHC II-C载体序列质粒序列多克隆位点信息
pEGFP-NMHC II-C质粒载体简介
pEGFP-NMHC II-C质粒序列载体序列
LOCUS pEGFP-NMHC II-C 10708 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pEGFP-NMHC II-C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10708)
AUTHORS Golomb E, Ma X, Jana SS, Preston YA, Kawamoto S, Shoham NG, Goldin
E, Conti MA, Sellers JR, Adelstein RS
TITLE Identification and characterization of nonmuscle myosin II-C, a new
member of the myosin II family.
JOURNAL J Biol Chem. 2004 Jan 23. 279(4):2800-8.
PUBMED 14594953
REFERENCE 2 (bases 1 to 10708)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10708
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 664..673
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 6655..6664
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 6661..7380
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(6706..6727)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(6967..6986)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 7314..7335
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 7503..7624
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7540..7559)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7594..7613
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(7631..8086)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7768..7789)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 7980..7999
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 8113..8217
/gene="bla"
/label=AmpR promoter
promoter 8219..8576
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(8242..8262)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 8427..8562
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8489..8508
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 8611..9405
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(8665..8684)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 9275..9294
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(9593..9612)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 9637..9684
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 10013..10601
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10502..10521
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtatcgat
661 gccgccacca tggctgcagt gaccatgtcc gtgtctggga ggaaggtagc ctccaggcca
721 ggcccggtgc ctgaggcagc ccaatcgttc ctctacgcgc cccggacgcc aaatgtaggt
781 ggccctggag ggccacaggt ggagtggaca gcccggcgca tggtgtgggt gccctcggaa
841 ctgcatgggt tcgaggcagc agccctgcgg gatgaagggg aggaggaggc agaagtggag
901 ctggcggaga gtgggcgccg cctgcggctg cccagggacc agatccagcg catgaaccca
961 cccaagttca gcaaggcaga agatatggct gagctcacct gcctcaacga ggcctcggtc
1021 ctgcacaacc tgcgagaacg ctactactcc gggctcattt atacctactc tggcctcttc
1081 tgtgtggtca ttaacccata caagcagctg cccatctaca cggaggccat tgttgaaatg
1141 taccggggca agaagcgcca tgaggtgcca cctcacgtgt atgctgtgac ggagggcgcg
1201 taccgcagca tgcttcagga tcgtgaggat caatccattc tctgcacggg agagtctggc
1261 gctgggaaga cggagaacac caagaaggtc atccagtacc tggcccatgt ggcatcatct
1321 ccaaagggca ggaaggagcc tggtgtccct ggggagctag agcgtcagct tcttcaagcc
1381 aaccccatcc tagaggcctt tggcaatgcc aagacagtga agaacgacaa ctcttcccga
1441 tttggcaaat tcatccgcat caactttgat attgctggct acatcgtggg agcaaacatc
1501 gagacctatc tgttggagaa gtcccgggcc atcagacagg ccaaggatga atgcagcttc
1561 catatcttct accagctgct agggggcgct ggggagcagc taaaagctga cctccttctg
1621 gagccctgtt cccattatcg cttcctgacc aatgggccct catcgtcccc gggccaggag
1681 cgtgagttat tccaggagac cctggagtcc ctgcgtgtgc tgggcctcct cccagaagag
1741 atcactgcca tgctgcgcac tgtctctgct gtcctccagt ttggcaacat tgtcctgaag
1801 aaagagcgca atacggacca agccaccatg cctgacaaca cagctgccca gaagctttgc
1861 cgcctcttgg gactcggagt gaccgacttc tccagagccc ttctcacacc ccgcatcaaa
1921 gtgggccgag attatgttca gaaagcacaa accaaggagc aggctgactt tgcgctggag
1981 gctctggcca aagctaccta tgagcgcctg ttccgctggc tggttctgcg gctcaaccgt
2041 gccctggaca gaagcccgcg gcagggtgcc tccttcctgg gcatcctgga catcgcgggc
2101 tttgagatct tccagctgaa ctccttcgag cagctgtgca tcaactacac caacgagaag
2161 ctacagcagc tattcaacca caccatgttc gtgctggagc aggaggagta ccagcgagag
2221 ggcatcccct ggaccttcct agacttcggg ttggacctgc aaccttgcat cgacctcatt
2281 gagcgtccgg ccaaccctcc aggtctcctg gccctgctgg acgaggagtg ctggttcccc
2341 aaggccacgg acaagtcttt tgtggagaag gtcgcccagg agcagggcag ccaccccaaa
2401 ttccagcgcc ccaggaacct gcgagatcag gccgacttca gcgtcctgca ctacgccggc
2461 aaggttgact acaaagccag tgagtggctg atgaagaaca tggacccact gaatgacaat
2521 gtggccgcct tgcttcacca gagcacggat cgtctcacag ctgagatctg gaaggatgtg
2581 gagggcatcg tggggctgga gcaagtaagc agccttggag atggcccacc gggaggccgc
2641 ccccgccgtg gaatgttccg gactgtgggg cagctctaca aagaatccct gagccgcctc
2701 atggccacgc tcagcaacac caaccctagt tttgtccgct gcatcgttcc caatcatgag
2761 aagagggctg gaaagctgga gccgcgcctg gtgctggacc aactccgttg taacggggtc
2821 ctcgagggta tacgcatctg tcgccaaggc ttccccaacc gcatcctctt ccaggagttc
2881 cgacagcgct atgaaatcct caccccgaac gctattccca agggcttcat ggacggcaaa
2941 caggcctgtg agaagatgat ccaggccctg gagctagacc ccaacctgta ccgtgttggc
3001 caaagcaaga tcttcttccg ggcaggggtc ctggcccagc tggaggagga gcgggacctg
3061 aaagtcaccg acatcatagt gtctttccag gcagcggcac ggggctacct ggcccgtagg
3121 gctttccaga gacggcagca gcagcagagt gctctgaggg tgatgcagag aaactgtgct
3181 gcctacctca agctcaggaa ctggcagtgg tggaggctgt tcatcaaggt gaagcccctg
3241 ctgcaggtga cacggcagga tgaggtgctg caggcgcgcg cccaggagct gcagaaagtt
3301 caggagctgc agcagcagag cgctcgtgaa gtgggggaac tgcagggtcg agtggcacag
3361 ctagaggagg agcgcacgcg cctggctgag cagcttcgag cagaagccga gctctgctct
3421 gaggccgagg agacgcgggc gcgactggct gcccggaagc aggagctgga gctggtggtg
3481 acagagctgg aggcacgagt gggcgaggaa gaagagtgca gccggcagct gcagagtgag
3541 aagaagaggc tgcagcagca tatccaggag ctagagagcc acctggaagc tgaggagggt
3601 gcccggcaga agctacagct ggagaaggtg accacagagg ccaagatgaa gaaatttgag
3661 gaggacctgc tgctcctgga ggaccagaat tccaagctga gcaaggagcg gaggctgctg
3721 gaggagcggc tggctgagtt ctcctcacag gcagcagaag aggaagagaa agtcaaaagt
3781 ctcaacaagc tgaggctcaa atatgaagcc acaatctcag acatggaaga ccggctgaag
3841 aaggaggaga agggacgcca ggaactagag aagctgaagc gacggctgga cggggagagc
3901 tcagagcttc aggagcagat ggtggagcag aagcagaggg cagaggaact gctcgcacag
3961 ctgggccgca aggaggatga gctgcaggcc gccctgctca gggcagagga agagggtggt
4021 gcccgtgccc agttgctcaa gtccctgcga gaggcacagg ctggccttgc tgaggctcag
4081 gaggacctgg aagctgagcg ggtagccagg gccaaggcgg agaagcagcg ccgggacctg
4141 ggcgaggagt tggaggccct acgtggggag ctcgaggaca ctctggattc caccaacgcc
4201 cagcaggagc tgcggtccaa gagggagcag gaggtgacag agctgaagaa agcattggaa
4261 gaggagtccc gtgcccatga ggtgtccatg caggagctga gacagaggca tagccaggca
4321 ctggtggaga tggccgagca gttggagcaa gcccggaggg gcaaaggtgt gtgggagaag
4381 actcggctat ccctggaggc tgaggtgtcc gagctgaagg ccgagctgag cagcctgcag
4441 acctcgagac aggagggtga gcagaagagg cgccgcctgg agtcccagct acaggaggtc
4501 cagggccgat ccagtgattc ggagcgggct cggtctgagg ctgctgagaa gctgcagaga
4561 gcccaggcgg aacttgagag cgtgtccaca gccctgagtg aggcggagtc caaagccatc
4621 aggctgggca aggagctgag cagtgcagag tcccagctgc atgacaccca ggaactgctt
4681 caggaggaga ccagggcaaa gctggccttg gggtcccgtg tgcgtgccct agaggccgag
4741 gcggcggggc ttcgggagca gatggaagag gaggtggttg ccagggaacg ggctggccgg
4801 gagctgcaga gcacgcaggc ccagctctct gaatggcggc gccgccagga agaagaggct
4861 gcggtgctgg aggctgggga ggaggctcgg cgccgtgcag cccgggaggc agagaccctg
4921 acccagcgcc tggcagaaaa gactgaggct gtagaacgac tggagcgagc ccggcgccga
4981 ctgcagcagg agttggacga tgccactgtg gatctggggc agcagaagca gctcctgagc
5041 acactggaga agaagcagcg gaaatttgac cagctcctgg cagaggagaa ggctgcagtt
5101 ctacgggctg tggaagaccg tgaacggata gaggccgaag gccgggagcg agaggcccgg
5161 gccctgtcgc tgacccgggc cctggaagag gagcaggagg cccgggagga gctggagagg
5221 cagaaccgtg ctctgagggc tgagctggaa gcactgctga gcagcaagga tgacgtgggc
5281 aagaacgtgc acgagctgga gcgagcccgt aaggcggctg aacaggcagc cagtgacctg
5341 cggacacagg tgacagaatt ggaggatgag ctgacagccg cagaggatgc caagctgcgc
5401 ctggaggtga ctgtgcaggc tctgaaggct caacatgaac gcgacctgca gggccgcgat
5461 gatgccggtg aggagaggcg gaggcagctg gccaagcagc taagagacgc agaggtagag
5521 cgcgatgagg aacggaagca gagggcactg gctatggctg cccgcaagaa gctggagctg
5581 gaactggagg agttgaaggc gcagacatct gctgctgggc agggcaagga agaggcagtg
5641 aagcagctga agaagatgca ggtccagatg aaggagctgt ggcgggaggt agaggagacg
5701 cgtagctccc gcgacgagat gtttaccctg agcagggaaa atgagaagaa gctcaagggg
5761 ctggaagctg aggtgctgcg tctgcaagag gaacttgctg cctcagaccg agcccggagg
5821 caggcccagc aagacagaga cgagatggca gaggaggtgg ccagtggcaa tcttagcaag
5881 gcagccaccc tggaggaaaa acggcagctg gaggggcgac tgagccagtt ggaagaggag
5941 ctggaggaag aacagaacaa ctcggagctg ctcaaggacc attaccgaaa gctagtgcta
6001 caggtcgagt ccctcaccac agaactgtct gccgaacgaa gtttctcagc caaggccgag
6061 agtggacggc agcagctgga gcggcagatc caggaactgc gggcccgctt gggtgaagag
6121 gatgctggag cccgagccag gcagaaaatg ctgatcgctg ctctggagtc taaactggcc
6181 caggcagagg agcagctgga gcaggagagc agggagcgca tcctctctgg caagctggta
6241 cgcagagctg agaagcggct gaaggaggta gttcttcagg tggatgaaga gcgcagggtg
6301 gctgaccagg tccgggacca gctggagaaa agcaacctcc ggctgaagca gctcaagagg
6361 cagctggagg aggcagagga ggaggcatct cgggcacagg ctggtcggag gcggctgcag
6421 cgggagctgg aggacgtcac tgagtctgca gaatccatga accgggaggt gaccacgctg
6481 aggaacaggc tccggcgtgg cccacttaca ttcaccacac ggactgtgcg ccaggtgttc
6541 cggctggaag agggcgtggc ttctgacgag gaagaggctg aaggagctga acctggctct
6601 gcaccaggcc aggagccgga ggctccgccc cctgccacac cccaaggatc catcgccacc
6661 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
6721 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
6781 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
6841 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
6901 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
6961 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
7021 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
7081 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
7141 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
7201 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
7261 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
7321 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
7381 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
7441 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
7501 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
7561 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
7621 cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
7681 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
7741 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
7801 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
7861 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
7921 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
7981 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
8041 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
8101 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
8161 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct
8221 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
8281 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
8341 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
8401 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
8461 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
8521 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc
8581 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
8641 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
8701 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
8761 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
8821 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
8881 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
8941 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
9001 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
9061 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
9121 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
9181 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
9241 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
9301 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
9361 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
9421 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
9481 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
9541 agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac
9601 ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa
9661 cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
9721 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
9781 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
9841 ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat
9901 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
9961 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
10021 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
10081 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
10141 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
10201 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
10261 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
10321 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
10381 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
10441 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
10501 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
10561 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
10621 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
10681 ctgattctgt ggataaccgt attaccgc
//
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pEGFP-NMHC II-C质粒载体应用举例
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